CD4 anticorps (N-Term) (APC)

N° du produit ABIN120587

L’anticorps Souris Monoclonal anti-CD4 a été validé pour FACS. Il convient pour détecter CD4 dans des échantillons de Humain. Il y a 1 publication disponible.

Aperçu rapide pour CD4 anticorps (N-Term) (APC) (ABIN120587)

Antigène: CD4

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD4 est conjugé à/à la APC

Application: Flow Cytometry (FACS)

Clone: MEM-241

Détail du produit anti-CD4 anticorps (APC)

Épitope: N-Term

Specificité: The antibody recognizes CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes (helper T-cells) and also on monocytes, tissue macrophages and granulocytes.

Purification: Size-exclusion chromatography

Immunogène: 2 N-terminal domains of human CD4 fused to human IgG1 Fc

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry (use 20 μL to label 10^6 cells or 100 μL whole blood).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Buffer: Phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store the antibody at 2 - 8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Shelf Life: one year from despatch.

Date de péremption: 12 months

Références pour anticorps anti-CD4 (APC) (ABIN120587)

Cole, Foti, Brown: "Twenty Years of Change on Campsites in the Backcountry of Grand Canyon National Park." dans: Environmental management, (2008) (PubMed).

Détails sur CD4

Antigène: CD4

Autre désignation: CD4

Sujet: CD4 is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1), IL-16 (binds to CD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin Intracellular ligands: p56Lck CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus, CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

ID gène: 920

UniProt: P01730

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events